Pcpgfree-mcs
SpletNucleic Acids Research Advance Access published January 20, 2015 Nucleic Acids Research, 2015 1 doi: 10.1093/nar/gkv008 A KALA-modified lipid nanoparticle containing CpG-free plasmid DNA as a potential DNA vaccine carrier for antigen presentation and as an immune-stimulative adjuvant Splet01. nov. 2013 · To prepare pDNA encoding luciferase that is completely free from unmethylated CpG dinucleotide motifs (pCpGfree-Luc (0)), the multiple cloning site of pCpGfree-mcs (Invivogen, San Diego, CA, USA) was preliminarily replaced with a new one that can be digested by 5′-Bgl II-Pvu II-Nco I-Sca I-Xba I-Nhe 1-3′ by ligating the hybridized …
Pcpgfree-mcs
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Splet18. okt. 2016 · CpG carrying versions of the promoter was cloned from the pCpGrich-mcs vector (InvivoGen, San Diego, CA) and the CpG free version was cloned from pCpGfree … Splet01. nov. 2024 · CpG-free and non-coding pDNA (pCpGfree-mcs) was used in this study. In this study, we attempted to develop an immune-stimulative adjuvant that activates a sequence of cytoplasmic DNA-sensing pathways (i.e. the cGAMP and subsequent STING/TBK1/IRF3 pathway 28, 29 ).
Splet28. feb. 2015 · To prepare pDNA encoding luciferase that is completely free from unmethylated CpG dinucleotide motifs (pCpGfree-Luc(0)), the multiple cloning site of pCpGfree-mcs (Invivogen, San Diego, CA, USA) was preliminarily replaced with a new one that can be digested by 5′-Bgl II-Pvu II-Nco I-Sca I-Xba I-Nhe 1-3′ by ligating the hybridized ... SpletExpression vector CpG-free selectable in mammalian cells - Blasticidin
SpletpCpGfree-vitro is a new family of expression vectors completely devoid of CpG dinucleotides that are selectable in mammalian cells. Similarly to the other pCpGfree … SpletCpGs) and a pCpGfree-mcs vec- tor encoding the CpG-positive luciferase gene (pCpGfree- Luc; 93 CpGs). The removal of the CpG sequences from the backbone and/or the ORF …
Splet3’) was cloned into a CpG-free vector (pCpGfree-mcs Invivogen, San Diego, CA, USA) using BglII and NcoI restriction endonuclease sites (New England Biolabs Ltd., Pickering, ON, Canada). The gene was designed such that it had minimal CpG oligodeoxynucleotides and was optimized for high expression in
SpletpCpGfree is a family of plasmids completely devoid of CpG dinucleotides. Typically, the elements required for replication and selection of the plasmid in E. coli and gene … Cloning & Expression Vectors. InvivoGen provides a broad range of cloning and ex… Custom Services InvivoGen provides two custom services: Immunomodulatory Co… NLRP1 & NLRP3: a tale of two inflammasome sensors . In this first newsletter of 2… E.coli Competent Cells. InvivoGen provides two different E. coli competent strains… Description. CpG-free Plasmid Backbone pCpGfree is a family of plasmids comple… unfollow vs block on facebookSpletA cloning vector completely devoid of CpG dinucleotides unfollow vs leave groupSpletpCpGfree-mcs. Similar products. Human MCS CRISPR Knock Out 293 Cell Line. Catalog number: K1282152. Size: 1x10^6 cells/1.0ml. Price: Please ask. Human MCS CRISPR Knock Out A549 Cell Line. Catalog number: K1282153. Size: 1x10^6 cells/1.0ml. Price: Please ask. Human MCS CRISPR Knock Out HeLa Cell Line. unfollow websiteSpletRegarding the non-coding pDNA, pCpG-free-MCS (Invivogen, San Diego, CA, USA) was used. ... Toxoplasma gondii GRA15 DNA Vaccine with a Liposomal Nanocarrier Composed of an SS-Cleavable and... unfollow wranglerSpletThe hHGF expression vector without any CpG, pCpGfree-hHGF was custom-made by subcloning the CpG-depleted hHGF gene into the pCpGfree-mcs vector (InvivoGen, San Diego, CA, USA). pCMV-Luc and pZsGreen1-N1 (Takara Bio Inc., Shiga, Japan) were amplified in the Escherichia coli strain DH5. pCpGfree-LacZ (InvivoGen, San Diego, CA, … unfollow webSplet01. avg. 2014 · Hyde et al. reported that the complete deletion of the unmethylated CpG dinucleotide (CpG) motifs from the pDNA conferred a sustained gene expression with minimal inflammatory response in the lung after in … unfollowbatchedSpletSpeI and BglII and inserted into pCpGfree-mcs vector. This final construct is hereinafter called p-εRNA. Figure 1. A plasmid map of U6 promoter-driven εRNA expression vector (p-εRNA) 2. The preparation of the p-εRNA loaded in a … unfollowable